Adenovirus Purification Kit - K1459

K1459 | Adenovirus Purification Kit DataSheet

’s Adenovirus Purification Kit is used to capture, concentrate and store adenoviruses using magnetic beads. Viruses are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally viruses are purified by ultracentrifugation using CsCl which is time consuming and limited to the volume of cell lysate to be processed. ’s Purification kit uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation. The viruses captured on Adenovirus (AD)-magnetic beads can be used directly for multiple assays. Alternatively, viruses can be eluted with the elution buffer and used for downstream applications. The AD-magnetic beads can be combined with any adenoviruses such as Ad Type 5 (Ad5), Oncolytic Ad (Ad520).

Alternate Name : Magnetic Virus Purification Kit, Virus purification Kit, Magnetic Beads Based Virus Purification kit, Virus Capture Kit, Adenovirus Purification Kit

Tag Line : Magnetic beads based kits

Summary : ’s Adenovirus Purification Kit is used to capture, concentrate and store adenoviruses using magnetic beads. Viruses are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally viruses are purified by ultracentrifugation using CsCl which is time consuming and limited to the volume of cell lysate to be processed. ’s Purification kit uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation. The viruses captured on Adenovirus (AD)-magnetic beads can be used directly for multiple assays. Alternatively, viruses can be eluted with the elution buffer and used for downstream applications.

Detection Method : Magnetic beads based virus purification

Sample Type : Cell and tissue culture supernatants, Serum

Species Reactivity : All

Applications : PCR, western blot, ELISA, transfection directly with viruses bound to magnetic beads or eluted from the magnetic beads (free of beads).

Features and Benefits : Does not need ultracentrifugation, Purify viruses in 30-45 min, High yield and fast concentration (2 to 1000X), Ideal for cell culture transduction/infection