ADPRHL2 Antibody (N-term) [APR32732G]

ADPRHL2 Antibody (N-term) [APR32732G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ADPRHL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-114 amino acids from the N-terminal region of human ADPRHL2.

Cellular Localisation: Nucleus. Cytoplasm. Chromosome. Mitochondrion matrix Note=Recruited to DNA lesion regions following DNA damage; ADP-D- ribose-recognition is required for recruitment to DNA damage sites

Molecular Weight: 38947

Clone: Polyclonal

Gene Name: ADPRS (HGNC:21304)

Gene ID: 54936

Function: ADP-ribose glycohydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP- ribose and acts on different substrates, such as proteins ADP- ribosylated on serine, free poly (ADP-ribose) and O-acetyl-ADP-D-ribose (PubMed:21498885, PubMed:30045870, PubMed:29907568, PubMed:30401461, PubMed:33186521) . Specifically acts as a serine mono-ADP- ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response (PubMed:28650317, PubMed:29234005, PubMed:30045870, PubMed:33186521) . Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:29480802, PubMed:33186521) . Does not hydrolyze ADP-ribosyl- arginine, -cysteine, -diphthamide, or -asparagine bonds (PubMed:16278211) . Also able to degrade protein free poly (ADP-ribose), which is synthesized in response to DNA damage: free poly (ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly (ADP-ribose) -dependent cell death, a process named parthanatos (PubMed:16278211) . Also hydrolyzes free poly (ADP- ribose) in mitochondria (PubMed:22433848) . Specifically digests O- acetyl-ADP-D-ribose, a product of deacetylation reactions catalyzed by sirtuins (PubMed:17075046, PubMed:21498885) . Specifically degrades 1''- O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D-ribose or 3''-O-acetyl-ADP-D-ribose isomers (PubMed:21498885) .

Summary: Tissue Location: Ubiquitous..

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC

Dilution: WB--1:1000 FC--1:10~50

Synonyms: Poly (ADP-ribose) glycohydrolase ARH3, ADP-ribosylhydrolase 3, [Protein ADP-ribosylarginine] hydrolase-like protein 2, ADPRHL2, ARH3