AF/LE Purified Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] | E-AB-F11120

AF/LE Purified Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] | E-AB-F11120 | Gentaur UK, US & Europe Distribution

Category: Flowcytometry Antibody

Synonyms: B220,CD45,GP180,LCA,T200

Swissprot Code: P06800,P08575

Application: Cell Sep-Neg, Depletion, FCM, IHC, IP

Reactivity: Mouse

Host: Rat

Isotype: Rat IgG2a, κ

Research Areas: N/A

Backgroun: CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-2CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.40 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources.

Concentration: 0.5 mg/mL

Storage: Store at 2~8°C. Do not freeze.

Immunogen: N/A

Buffer: 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.

Purification Method: N/A

Dilution: N/A

Clone: RA3.3A 1/6.1

Conjugation: None (Purified antibody-Azide Free/Low endotoxin)

Moleculair Weight(Calculated): N/A

Moleculair Weight(Observed): N/A