AGO10 | Argonaute 10 | AS15 3071

AGO10 | Argonaute 10 | AS15 3071 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO10 protein sequence, Uniprot: Q9XGW1, TAIR: AT5G43810

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

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Recommended Dilutions: 1 : 10 000 (WB)

Molecular weight: 110, 9 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: A. lyrata, B. napus, C. rubella, C. clementina, C. sinensis, E. salsugineum, G. arboreum, G. raimondii. N. benthamianaSpecies of your interest not listed? Contact us

Not reactive in: Zea mays

Additional Information: AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

Background: AGO10 is involved in miRNA binding, and in RNA-mediated posttranscriptional gene silencing (PTGS) .

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G43810

Category: microRNA

Research Area: RNAi/siRNA/miRNA