AGO2 | Argonaute 2 | AS13 2682

AGO2 | Argonaute 2 | AS13 2682 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified IgY in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunoprecipitation (IP), Western blot (WB)

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Recommended Dilutions: 1 : 250-1 : 500 (WB)

Molecular weight: 113 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Capsella rubella, Solanum tuberosum Species of your interest not listed? Contact us

Not reactive in: Chlamydomonas reinhardtii, Nicotiana benthamiana, Solanum lycopersicum, Zea mays

Additional Information: AGO2 protein is strongly induced by stress. AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Antibody incubation should be done over night in 4°C. Use of material with enriched AGO2 levels is recommened.

Background: AGO2 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC) . This protein complex is responsible for the gene silencing (RNAi) . AGO2 is probably involved in antiviral RNA silencing.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G31280

Category: Pathogen attack

Research Area: Plant Biology