AIRE Antibody | 45-225

AIRE Antibody | 45-225 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: N/A

Immunogen: The immunogen for this antibody is: C-QSMARPAAPFPS

Research Area: Cancer

Tested Application: E, WB, IHC, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary testing showed an approx 60kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7kDa according to NP_000374.1) . Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Smooth Muscle lysate. This product has been successfully used in Western blot by an anonymous customer on HEK293 cell lysate.Immunohistochemistry:In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: Cat. No. 1210 - 293 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy) , APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein) , autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) , autoimmune regulator, AIRE1, OTTHUMP00000109529, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy

User Note: N/A

BACKGROUND: N/A