AKR1C3 protein (His tag) | 80R-1116

AKR1C3 protein (His tag) | 80R-1116 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Conjugated Proteins

Research Area: Cytokines & Growth Factors

Short Description: Purified recombinant Human AKR1C3 protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: 1-323 amino acids: MGSSHHHHHH SSGLVPRGSH MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY

Tag/conjugate: His tag

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 95% pure

Methode of Purification: N/A

Source: N/A

Concentration: 1 mg/ml

Form & Buffer: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.

Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: SDS-PAGE

Bioactivity: Specific activity: approximately < 0.1 units/mg. Ezymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 Deg C.

Usage Recommendations: N/A

Assay Information: 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1C3 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.