26
Akt Negative Control Cell Lysate | 7035
- SKU:
- 26-7035-GEN
- Availability:
- Usually shipped in 5 working days
Description
Jurkat cells growing in DMEM supplemented with 10% FBS were collected and washed in ice-cold PBS. Cells were then lyzed in three volume of Kinase Extraction Buffer (70002-N80M in K435-40), keep on ice for 5 min, centrifuge at top speed for 15 min. Collect the cell lysate. Extract the pellet again with one volume Kinase Extraction Buffer. Combine the cell lysate together, determine protein concentration. The cell lysate can be used as a negative control for Akt activity assays, in conjunction with the use of ’s Akt Activity Assay Kit (K435-40). We recommend using 100 µg per assay.
7035 | Akt Negative Control Cell Lysate DataSheet
Sort Name: Akt Negative Control Cell Lysate
Label Name: N/A
Taglines: Uninduced Jurkat cell extracts useful as a control in western blotting or activity assays.
Product Highlights: • CONCENTRATION- 4 mg/ml
Packaging: EA
Additional Information
Storage Temperature: |
-70°C |
Shipping: |
dry ice |
Shelf Life: |
6 months |
5 Reviews
-
Speedy delivery
as compared to its competitors, they deliver their products very quickly. Highly appreciatable their speedy delivery
-
perfect
never found any mess with them always found them very supportive and friendly. In short, Gentaur is perfect
-
Excellent
the job Gentaur is doing and tackling with its competitors is excellent
-
outstanding
I ordered the 156G recombinant and the result of that product was outstanding. Really impressed
-
good support
for company self-esteem, good support is necessary and gentaur is highly appreciatable for its good support