AKT1 / AKT2 / AKT3 (Ab 315 / 316 / 312) Antibody | 79-417

AKT1 / AKT2 / AKT3 (Ab 315 / 316 / 312) Antibody | 79-417 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: AKT1/AKT2/AKT3 (Ab-315/316/312) antibody was raised against a peptide sequence around aa.313~317/314~318/310~314 (P-E-Y-L-A) derived from Human AKT1/AKT2/AKT3.

Research Area: Other

Tested Application: IHC

Application: Immunohistochemistry: 1:50~1:100

Specificiy: This antibody detects endogenous level of total AKT1/AKT2/AKT3 protein.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 60 kDa

Validation: N/A

Isoform: N/A

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+) , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/mL

Storage Condition: Store antibody at -20˚C for up to one year.

Alternate Name: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, P53, RAC-PK-alpha, Protein kinase B

User Note: N/A

BACKGROUND: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF) , epidermal growth factor (EGF) , insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. /IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.