AKT1 Antibody | 22-824

AKT1 Antibody | 22-824 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human AKT1 (NP_005154.2) .

Research Area: Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction

Tested Application: WB, IP

Application: WB: 1:500 - 1:2000
IP: 1:20 - 1:50

Specificiy: N/A

Positive Control 1: NCI-H460

Positive Control 2: MCF7

Positive Control 3: Mouse kidney

Positive Control 4: Rat heart

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Observed: 57kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: Akt1 Antibody: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, Protein kinase B

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.