AKT1 Antibody| F40742-0.08ML/F40742-0.4ML/R20273-0.1ML/R30075/V3797-20UG

AKT1 Antibody| F40742-0.08ML/F40742-0.4ML/R20273-0.1ML/R30075/V3797-20UG | Gentaur UK, US & Europe Distribution

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

Format: Antigen affinity purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: Bovine, Mouse, Rat, Xenopus

Application: IHC, FACS, WB, ELISA

Application Details: Western blot: 1:1000, IHC (Paraffin): 1:50-1:100, Flow Cytometry: 1:10-1:50

Application Note: Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This AKT1 antibody is available for research use only.

Purity: Antigen affinity

Immunogen: A portion of amino acids 282-309 from the human protein was used as the immunogen for this AKT1 antibody.

Storage: Aliquot the AKT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A