Additional Information
Size:  | 
                                                    100 µl  | 
                                                
Target:  | 
                                                    AKT1 (pThr308)  | 
                                                
Conjugate:  | 
                                                    Dylight 350  | 
                                                
Research Area:  | 
                                                    Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | PKB / AKT | Cancer | Apoptosis | Cell Signaling | Metabolism | Metabolism processes  | 
                                                
Alternative Name:  | 
                                                    AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody  | 
                                                
Category:  | 
                                                    Antibodies  | 
                                                
Product Type:  | 
                                                    Polyclonal Antibody  | 
                                                
Immunogen:  | 
                                                    A phospho-specific peptide corresponding to residues surrounding Thr308 of human Akt1 (AA305-311)  | 
                                                
Immunogen Species:  | 
                                                    Human  | 
                                                
Applications:  | 
                                                    WB, AM  | 
                                                
Host:  | 
                                                    Rabbit  | 
                                                
Isotype:  | 
                                                    N/A  | 
                                                
Species Reactivity:  | 
                                                    Human  | 
                                                
Antibody Dilution:  | 
                                                    WB (1:250); optimal dilutions for assays should be determined by the user.  | 
                                                
Purification:  | 
                                                    Peptide Affinity Purified  | 
                                                
Storage Buffer:  | 
                                                    PBS pH7.4, 50% glycerol, 0.025% Thimerosal  | 
                                                
Concentration:  | 
                                                    N/A  | 
                                                
Specificity:  | 
                                                    Detects 55.686 kDa.  | 
                                                
Storage:  | 
                                                    -20ºC  | 
                                                
Shipping:  | 
                                                    Blue Ice or 4ºC  | 
                                                
Certificate of Analysis:  | 
                                                    A 1:250 dilution of SPC-910 was sufficient for detection of AKT1 (pThr308) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.  | 
                                                
Cellular Localization:  | 
                                                    Cytoplasm, Nucleus, Cell Membrane  | 
                                                
Tissue Specificity:  | 
                                                    Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer  | 
                                                
Accession Number:  | 
                                                    NP_005154  | 
                                                
Gene ID:  | 
                                                    207  | 
                                                
Swiss-Prot:  | 
                                                    P31749  | 
                                                
Field of Use:  | 
                                                    For in vitro research use only.  |