Additional Information
Size: |
100 µl |
Target: |
AKT1 (pTyr315) |
Conjugate: |
Dylight 488 |
Research Area: |
Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | PKB / AKT | Cancer | Apoptosis | Cell Signaling | Metabolism | Metabolism processes |
Alternative Name: |
AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
A phospho-specific peptide corresponding to residues surrounding Tyr315 of human Akt1 (AA312-318) |
Immunogen Species: |
Human |
Applications: |
WB, AM |
Host: |
Rabbit |
Isotype: |
N/A |
Species Reactivity: |
Human |
Antibody Dilution: |
WB (1:250); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS pH7.4, 50% glycerol, 0.025% Thimerosal |
Concentration: |
N/A |
Specificity: |
Detects 55.686 kDa. |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:250 dilution of SPC-911 was sufficient for detection of AKT1 (pTyr315) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Cellular Localization: |
Cytoplasm, Nucleus, Cell Membrane |
Tissue Specificity: |
Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer |
Accession Number: |
NP_005154 |
Gene ID: |
207 |
Swiss-Prot: |
P31749 |
Field of Use: |
For in vitro research use only. |