Additional Information
| Size: | 100 µl | 
| Target: | AKT1 (pTyr315) | 
| Conjugate: | Unconjugated | 
| Research Area: | Cell Signaling | Protein Phosphorylation | Serine / Threonine Kinases | PKB / AKT | Cancer | Apoptosis | Cell Signaling | Metabolism | Metabolism processes | 
| Alternative Name: | AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody | 
| Category: | Antibodies | 
| Product Type: | Polyclonal Antibody | 
| Immunogen: | A phospho-specific peptide corresponding to residues surrounding Tyr315 of human Akt1 (AA312-318) | 
| Immunogen Species: | Human | 
| Applications: | WB, AM | 
| Host: | Rabbit | 
| Isotype: | N/A | 
| Species Reactivity: | Human | 
| Antibody Dilution: | WB (1:250); optimal dilutions for assays should be determined by the user. | 
| Purification: | Peptide Affinity Purified | 
| Storage Buffer: | PBS pH7.4, 50% glycerol, 0.025% Thimerosal | 
| Concentration: | N/A | 
| Specificity: | Detects 55.686 kDa. | 
| Storage: | -20ºC | 
| Shipping: | Blue Ice or 4ºC | 
| Certificate of Analysis: | A 1:250 dilution of SPC-911 was sufficient for detection of AKT1 (pTyr315) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. | 
| Cellular Localization: | Cytoplasm, Nucleus, Cell Membrane | 
| Tissue Specificity: | Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancer | 
| Accession Number: | NP_005154 | 
| Gene ID: | 207 | 
| Swiss-Prot: | P31749 | 
| Field of Use: | For in vitro research use only. | 
 
             
             
             
             
             
             
             
             
             
            