AKT1 (Phospho-T450) polyclonal Antibody | BS94050

AKT1 (Phospho-T450) polyclonal Antibody | BS94050 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Application: WB IHC IP

Application Range: WB:1:1,000-1:2,000 IHC:1:50-1:100

Background: The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s) . The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: AKT1 (Phospho-T450) polyclonal Antibody detects endogenous levels of AKT1 protein only when phosphorylated at T450.

Molecular Weight: 56 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: PKB, PKB alpha, AKT1, AKT 1, AKT, AKT1, AKT1, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein Kinase B Alpha, Protein kinase B, Proto-oncogene c-Akt, RAC Alpha, RAC, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr450 of human AKT1.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: ProA affinity purified

Pathway: