Akt2, Active | 7702

Akt2 or Protein Kinase B (PKB) is a serine/threonine kinase that is a member of the Akt family. Akt2 like the other Akt members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3, 4, 5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2. Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (3).

7702 | Akt2 Active DataSheet

Biomolecule/Target: N/A

Synonyms: Akt, RAC-alpha serine/threonine-protein kinase

Alternates names: Akt, RAC-alpha serine/threonine-protein kinase

Taglines: An important signaling molecule in the Insulin signaling pathway

NCBI Gene ID #: 20315

NCBI Gene Symbol: SDF1B

Gene Source: N/A

Accession #: P40224

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 46 nmol phosphate incorporated into Akt/SGK substrate (RPRAATF) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32.

Biological activity: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50-100 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 8.5 kDa

Concentration: 5 µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A