Akt3, Active | 7703

Akt3 or Protein Kinase B (PKB) is a serine/threonine kinase that is a member of the Akt family. In mammals, the Akt family comprises of three highly homologous members known as Akt1 (PKB), Akt2 (PKB), and Akt 3. Akt 3 like the other family members is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components. The activation mechanism remains to be fully characterized but occurs downstream of phosphoinositide 3-kinase (PI3K). PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt family members to the plasma membrane where they are phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) and phosphoinositide-dependent kinase-2 (PDK-2 possibly ILK). Akt 3 like the other family members phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that Akt 3 is frequently overexpressed in many types of human cancers including breast and prostate. Although the mechanisms have not yet been fully characterized, increased expression and activation of Akt is believed to promote cell proliferation and survival thereby contributing to cancer progression.

7703 | Akt3 Active DataSheet

Biomolecule/Target: N/A

Synonyms: Akt, RAC-alpha serine/threonine-protein kinase

Alternates names: Akt, RAC-alpha serine/threonine-protein kinase

Taglines: A serine/threonine-protein kinase

NCBI Gene ID #: 7133

NCBI Gene Symbol: TNFRII

Gene Source: N/A

Accession #: P20333

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 206 nmol phosphate incorporated into Akt substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32.

Biological activity: The ED50 as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 0.2 µg/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-a.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 20.0 kDa

Concentration: 5 µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Amino acid sequence: MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT.