Alanine Aminotransferase (ALT, GPT), Porcine Heart | P1298

The alanine aminotransferase (ALT) blood test is typically used to detect liver injury. GPT/ALT test is often ordered in conjunction with aspartate aminotransferase (AST) or as part of a liver panel to screen for and/or help diagnose liver disease. AST and ALT are considered to be two of the most important tests to detect liver injury, although ALT is more specific than AST. Sometimes AST is compared directly to ALT and an AST/ALT ratio is calculated. This ratio may be used to distinguish between different causes of liver damage. GPT values are often compared to the results of other tests such as alkaline phosphatase (ALP), total protein, and bilirubin to help determine which form of liver disease is present. ALT is often used to monitor the treatment of persons who have liver disease, to see if the treatment is working, and may be ordered either by itself or along with other tests for this purpose.

P1298 | Alanine Aminotransferase ALT GPT Porcine Heart DataSheet

Biomolecule/Target: Alanine Aminotransferase (ALT, GPT)

Synonyms: Glutamate Pyruvate Transaminase, Alanine Transaminase, serum glutamic-pyruvic transaminase, SGPT, GPT, ALT, ALAT

Alternates names: Glutamate Pyruvate Transaminase, Alanine Transaminase, serum glutamic-pyruvic transaminase, SGPT, GPT, ALT, ALAT

Taglines: It is helpful in diagnose liver disease.

NCBI Gene ID #: 7133

NCBI Gene Symbol: TNFRII

Gene Source: Pig

Accession #: P20333

Recombinant: No

Source: Porcine Heart

Purity by SDS-PAGEs: 97%

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED50 as determined by its ability to inhibit the TNF-a mediated cytotoxicity in the L-929 cells is less than 0.2 µg/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-a.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4.

Molecular Weight: 20.0 kDa

Concentration: N/A

Appearance: lyophilized

Physical form description: Lyophilized from tris-chloride, pH 7.0 with sodium chloride, EDTA, DTT, a-ketoglutarate and pyridoxal-5-phosphate.

Reconstitution Instructions: It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Amino acid sequence: MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT.