118
Aldose reductase protein | 30R-1234
- SKU:
- 118-30R-1234-GEN
- Availability:
- Usually ships in 5 working days
Description
Aldose reductase protein | 30R-1234 |
Activity Code: ACTIVE
Product Type: Proteins
Product Subtype: Recombinant Proteins
Research Area: Cell Cycle & Cell Death
Short Description: Purified recombinant Human Aldose reductase protein
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: Human
Residues: 1-316 amino acids: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
Tag/conjugate: N/A
Protein Type: Recombinant
Expression System: E.coli
Grade & Purity: > 95% pure
Methode of Purification: N/A
Source: N/A
Concentration: 1 mg/ml
Form & Buffer: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.
Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping Information: *Blue Ice*
Applications: SDS-PAGE
Bioactivity: Specific activity: approximately 0.5-0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH/min at 25 DEG C.
Usage Recommendations: N/A
Assay Information: 1. Prepare a 1ml reaction mix into a suitable container : The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1B1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25 DEG C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.