Alkaline phosphatase, placental, human recombinant | P1044

ALPP, also known as alkaline phosphatase, placental, is a family of dimeric metalloenzymes catalyzing the hydrolysis of monoesters of phosphoric acid. Also, this protein catalyzes a transphosphorylation reaction in the presence of large concentrations of phosphate acceptors. It occurs widely in nature, and are found in many organisms from Escherichia coli to man. Most alkaline phosphatases are homodimeric enzymes and each catalytic site contains three metal ions (two Zn and one Mg), that are necessary for enzymatic activity. Recombinant human ALPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

P1044 | Alkaline phosphatase placental human recombinant DataSheet

Biomolecule/Target: ALPP

Synonyms: ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

Alternates names: ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

Taglines: A hydrolase enzyme responsible for removing phosphate groups

NCBI Gene ID #: 10673

NCBI Gene Symbol: TN13B

Gene Source: Human

Accession #: Q9Y275

Recombinant: Yes

Source: Baculovirus

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): Measured by its ablity to hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Biological activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED for this effect is less than 10 ng/ml. Please note: results may vary with PBMC donors.

Results: > 2,500 units/mg

Binding Capacity: N/A

Unit Definition: Unit activity is defined as the amount of enzyme that hydrolyzes 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Molecular Weight: 19.335 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A