Alkaline Phosphatase Staining Kit - K2035

K2035 | Alkaline Phosphatase Staining Kit DataSheet

The Alkaline Phosphatase (AP) Staining Kit provides a quick and easy method to stain Alkaline Phosphatase in pluripotent stem cells. It is based on the ability of Alkaline Phosphatase to hydrolyze the phosphate group on the substrate, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) to form a blue colored intermediate. Nitro Blue Tetrazolium (NBT) then oxidizes the intermediate to form an insoluble, dark blue dimer. Because stem cells/pluripotent stem cells have increased AP activity, the AP Staining Kit can be used to identify and screen Alkaline Phosphatase activity in these cells. The staining method is simple, rapid, cost-effective and reliable. The entire procedure can be performed in the same microtiter plate and does not require any extra steps such as fixing or harvesting the cells. The assay can be also used as the initial step to test the pluripotency of cells and to monitor osteogenic differentiation of pluripotent/stem cells.

Alternate Name : Alkaline Phosphatase, Alkaline Phosphatase Kit, AP Staining, Phosphatase, Alkaline Phosphatase Staining Kit, AP Staining Kit, Alkaline Phosphatase Staining, Alkaline Phosphatase Kit

Tag Line : To identify and screen Alkaline Phosphatase activity in cells

Summary : The Alkaline Phosphatase (AP) Staining Kit provides a quick and easy method to stain Alkaline Phosphatase in pluripotent stem cells. It is based on the ability of Alkaline Phosphatase to hydrolyze the phosphate group on the substrate, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) to form a blue colored intermediate. Nitro Blue Tetrazolium (NBT) then oxidizes the intermediate to form an insoluble, dark blue dimer. Because stem cells/pluripotent stem cells have increased AP activity, the AP Staining Kit can be used to identify and screen Alkaline Phosphatase activity in these cells. The staining method is simple, rapid, cost-effective and reliable. The entire procedure can be performed in the same microtiter plate and does not require any extra steps such as fixing or harvesting the cells. The assay can be also used as the initial step to test the pluripotency of cells and to monitor osteogenic differentiation of pluripotent/stem cells.

Detection Method : Staining followed by microscopy

Sample Type : Induced pluripotent stem cells, Stem cells, Cell lines containing Alkaline Phosphatase activity

Species Reactivity : All

Applications : AP Staining in stem cells/pluripotent stem cells or stem cells undergoing osteogenesis., Screening for pluripotent cells induced by various factors., Detection of alkaline phosphate activity in stem cells/pluripotent stem cells, HeLa, HepG2, HEK293 cells, osteoblasts and osteocytes.

Features and Benefits : To identify and screen Alkaline Phosphatase activity in cells, Rapid, Cost-effective, Reliable, Simple