ALOX12 Antibody (C-term) [APR10855G]

ALOX12 Antibody (C-term) [APR10855G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M, Rat

Specificity: This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12.

Cellular Localisation: Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF

Molecular Weight: 75694

Clone: Polyclonal

Gene Name: ALOX12 (HGNC:429)

Gene ID: 239

Function: Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:17493578, PubMed:1851637, PubMed:8319693, PubMed:8500694, PubMed:18311922, PubMed:32404334) . Mainly converts arachidonate ( (5Z, 8Z, 11Z, 14Z) -eicosatetraenoate) to the specific bioactive lipid (12S) -hydroperoxyeicosatetraenoate/ (12S) -HPETE (PubMed:17493578, PubMed:22984144, PubMed:24282679, PubMed:8319693, PubMed:8500694) . Through the production of bioactive lipids like (12S) - HPETE it regulates different biological processes including platelet activation (PubMed:8319693, PubMed:8500694) . It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S) -hydroperoxy-docosahexaenoate/ (14S) -HPDHA resulting in the formation of (13S, 14S) -epoxy-DHA (PubMed:23504711) . Furthermore, it may participate in the sequential oxidations of DHA ( (4Z, 7Z, 10Z, 13Z, 16Z, 19Z) -docosahexaenoate) to generate specialized pro- resolving mediators (SPMs) like resolvin D5 ( (7S, 17S) -diHPDHA) and (7S, 14S) -diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334) . An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites (PubMed:8250832) . Can also peroxidize linoleate ( (9Z, 12Z) -octadecadienoate) to (13S) - hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity) . Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic (PubMed:9751607, PubMed:16638750, PubMed:22237009) . Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells (PubMed:23578768) .

Summary: Tissue Location: Expressed in vascular smooth muscle cells.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, IF, FC

Dilution: WB--1:1000 WB--1:2000

Synonyms: Arachidonate 12-lipoxygenase, 12S-type, 12S-LOX, 12S-lipoxygenase, Lipoxin synthase 12-LO, 332-, Platelet-type lipoxygenase 12, ALOX12, 12LO, LOG12