Alpha-Mannosidase Activity Assay Kit (Fluorometric) - K2041

K2041 | Alpha-Mannosidase Activity Assay Kit (Fluorometric) DataSheet

Alpha-Mannosidase (α-Man; EC 3.2.1.24) is a glycoside hydrolase involved in the cleavage of the alpha-mannose. α-Man can be applied in studies that determine the effects of mannose on specific molecules, such as recombinant proteins that are used for vaccine development. Lysosomal α-Man is essential for catabolizing the Asn-linked glycans of glycoproteins and is actively involved in maintaining the cellular homeostasis. α-Man deficiency results in Alpha-Mannosidosis, an acquired lysosomal storage disorder in which α-Man fails to break down mannose-containing oligosaccharides. These oligosaccharides, therefore, accumulate in lysosomes thereby leading to cellular malfunction and apoptosis. ’s Alpha-Mannosidase Activity Assay Kit provides a facile, rapid way to monitor total α-Man activity in various biological samples. In this kit, α-Man cleaves a synthetic specific substrate and releases a fluorophore, which can be easily quantified (Ex/Em = 360/445 nm). The substrate is specific to α-Man and can differentiate from β-mannosidase. The assay is simple, sensitive and can detect as low as 1.0 µU of alpha mannosidase activity.

Alternate Name : Alpha-Mannosidase Activity Assay Kit (Fluorometric),Alpha-Mannosidase Assay Kit (Fluorometric),Alpha-Mannosidase Assay Kit (Fluorometric),Alpha-Mannosidase,Mannosidase Activity Assay Kit (Fluorometric),α-Man,Alpha-Mannosidase Activity Assay (Fluorometric),EC 3.2.1.24,Alpha-Mannosidase Activity Kit,Alpha-Mannosidase Activity Assay Kit,Mannosidase Activity Assay,Alpha-Mannosidase Activity Assay,Mannosidase Activity Assay Kit,Alpha-Mannosidase Activity Assay

Tag Line : To monitor total α-Mannosidase activity

Summary : ’s Alpha-Mannosidase Activity Assay Kit provides a facile, rapid way to monitor total α-Man activity in various biological samples. In this kit, α-Man cleaves a synthetic specific substrate and releases a fluorophore, which can be easily quantified (Ex/Em = 360/445 nm). The substrate is specific to α-Man and can differentiate from β-mannosidase. The assay is simple, sensitive and can detect as low as 1.0 µU of alpha mannosidase activity.

Detection Method : Fluorescence (Ex/Em = 360/445 nm)

Sample Type : Tissue homogenates: Rat liver, etc.,Cell Lysates: 3T3 cells, etc.,Biological fluids: Human plasma, etc.

Species Reactivity : All

Applications : Measurement of alpha mannosidase activity in various samples

Features and Benefits : Can detect as low as 1.0 µU of alpha mannosidase activity.,A facile, rapid way to monitor total Alpha-Mannosidase in various biological samples.,The substrate is specific to α-Man and can differentiate from β-mannosidase.