AMDX

AmoyDx® KRAS/NRAS/BRAF Mut ations Detection Kit | 8.01.25403W006

(No reviews yet) Write a Review

SKU:: AMDX-AM-8.01.25403W006-GEN
UPC:
MPN:
Availability:: IN STOCK

£600.00

Current Stock:

Quantity:

Description
Additional Information

Description

AmoyDx® KRAS/NRAS/BRAF Mut ations Detection Kit | 8.01.25403W006 | Gentaur UK, US & Europe Distribution

KRAS, NRAS and BRAF gene are the key molecules in the downstream signaling pathway of epidermal growth factor receptor (EGFR), which transduce signals from membrane-bound receptors via multiple downstream effector pathways and thereby affect fundamental cellular processes, including proliferation, apoptosis, and differentiation. In total, activating KRAS and NRAS mutations occur in 20~50% and 1~6% of colorectal cancers respectively, mainly in exons 2, 3 or 4. And the most common BRAF mutations are V600 mutations in exon 15, which occur in 8〜15% of colorectal cancers.

Studies have shown the mutation status of the KRAS, NRAS and BRAF gene is relevant to the primary drug resistance of colorectal cancers treated with anti-EGFR monoclonal antibodies. Patients with wild-type KRAS and NRAS gene could benefit from Erbitux (Cetuximab) or Vectibix (Panitumumab), whereas, the patients with mutant KRAS or NRAS gene show poor response to this treatment. And BRAF gene mutations predict poor prognosis of such treatments. As a result, KRAS, NRAS and BRAF mutations detection supplies evidence for targeted clinical therapy of tumor patients, which decreases cost and time of treatment.

Intended Use

The AmoyDx® KRAS/NRAS/BRAF Mutations Detection Kit is a real-time PCR assay for qualitative detection of 17 KRAS mutations (exons 2, 3 and 4), 13 NRAS mutations (exons 2, 3 and 4) and BRAFV600E mutation (exon 15) in human genomic DNA extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue. The kit is intended to assess KRAS/NRAS/BRAF mutation status in colorectal cancer patient.

The kit is for in vitro diagnostic use, and intended to be used by trained professionals in a laboratory environment.

Testing Procedure