Amphiregulin, Human Recombinant | 7114

Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant human Amphiregulin is a 11.3 kDa glycoprotein consisting of 98 amino acid residues.

7114 | Amphiregulin Human Recombinant DataSheet

Biomolecule/Target: Amphiregulin

Synonyms: AR, AREG, Colorectum cell-derived growth factor (CRDGF)

Alternates names: AR, AREG, Colorectum cell-derived growth factor (CRDGF)

Taglines: Stimulates growth of keratinocytes, epithelial cells and some fibroblasts

NCBI Gene ID #: 115650

NCBI Gene Symbol: TR13C

Gene Source: Human

Accession #: Q96RJ3

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.

Biological activity: Determined by its ability to block BAFF induced mouse splenocyte survival. The expected ED for this effect is typically 1-5 µg/ml in the presence of 1 µg/ml human soluble BAFF.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 7.7 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Sodium phosphate, pH 7.0.

Reconstitution Instructions: Reconstitute in sterile HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A