Annexin V-AbFluor™ 405 Apoptosis Detection kit (Blue Fluorescence) | KTA0001

Annexin V-AbFluor™ 405 Apoptosis Detection kit (Blue Fluorescence) | KTA0001 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cellular states

Product Type: Apoptosis Detection Kit

Sequence: N/A

Activity: N/A

protein Lenght: N/A

Preparation: N/A

Purity: N/A

Formulation: N/A

Kit Component: • Annexin V Binding Buffer (5x)
• Annexin V- AbFluor™ 488
• Propidium Iodide (PI)

Features & Benefits : • Abbkine AbFluor™ 488 dye is superior to FITC as it is brighter, not affected by pH, and has much better photostability
• Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA)
• Suitable for flow cytometry or fluorescence microscopy

Molecular Weight: N/A

Usage Note: N/A

Storage Conditions: Stable for at least 6 months at 4°C from date of shipment. Protect from light and do not freeze.

Shipping: Blue ice

Background: Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS) . After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium iodide (PI) is a membrane-impermeant DNA-binding dye that is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red fluorescence.

Alternative Names: Annexin 5; Annexin V; AbFluor™ 488

Search Names: Annexin 5; Annexin V; AbFluor™ 488

Tag: Annexin V-AbFluor™ 488