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Anti 4-HNE monoclonal antibody

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SKU:
50-MHN-100P-GEN
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NULL870.00

Description

Anti 4-HNE monoclonal antibody

Description 4-hydroxy-2-nonenal (4-HNE):


Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies, and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation.

Specifications
Clone #: HNEJ-2
Immunogen: 4-HNE-modified Keyhole Limpet Hemocyanin
Subclass: Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified.
Form: Lyophilized Powder (containing 20 µg or 100 µg of IgG)
Specificity: This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
Applications: Immunohistochemistry and western blotting.
Storage: Less than -20°C for 5 years. When transport, stable at least 7 days at room temperature.

 

References
Immunohistochemistry:
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997)
Western blotting:
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995)
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Additional Information

Size:
100 ug of IgG/vial
Type of Marker:
Lipid oxidation
Marker:
ACR
Storage:
Less than -20°C
Usage:
Tissue
Application:
Immunohistochemistry, western blotting and ELISA.
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Anti 4-HNE monoclonal antibody (HNEJ2)
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Anti 4-HNE monoclonal antibody (HNEJ2)

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Anti 4-HNE monoclonal antibody (HNEJ2) Description 4-hydroxy-2-nonenal (4-HNE): Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies, and membrane lipids are one...
50-MHN-020P-GEN