Anti- Caspase 4 Antibody | FNab01291

Anti- Caspase 4 Antibody | FNab01291 | Gentaur UK, US & Europe Distribution

Form: liquid

Purification: Immunogen affinity purified

Purity: ≥95% as determined by SDS-PAGE

Host: Rabbit

Clonality: polyclonal

Clone ID: N/A

Isotype: IgG

Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: Inflammatory caspase, essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352) . Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:24879791, PubMed:25964352) . Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection (PubMed:25121752) . In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (PubMed:26508369) . Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains (PubMed:15123740, PubMed:22246630, PubMed:23661706) . Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034) . Does not directly process IL1B (PubMed:7743998, PubMed:7797592, PubMed:7797510) .

Immunogen: caspase 4, apoptosis-related cysteine peptidase

synonyms: CASP 4, CASP4, Caspase 4, Caspase4, ICE (rel) II, ICE (rel) II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX

Molecular weight (observed) : 43 kDa

Reactivity Species: Human

Tested Application: ELISA, IHC, WB, IF, IP

Recomended Dillution : WB: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:50-1:500

Research Area: Metabolism