Description

Anti Dibromo-Tyrosine (DiBrY) monoclonal antibody

A novel biomarker for protein oxidation

Neutrophils and eosinophils play an important role in the defence system against microbial infection. Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are known to catalyze formation of hypochlorous acid (HOCl) and hypobromous acid (HOBr). These reactive intermediates may react with proteins, lipids and nucleotides, and it has been reported to form tyrosine halogenation such as dibromotyrosine (DiBrY). DiBrY is a Br-modified tyrosine at 3- and 5- position, which is one of the major oxidative products derived from neutrophil myeloperoxidase.

Specific to dihalogenated tyrosine at 3- and 5-position

Monoclonal antibody clone 3A5 have been established by Dr. Toshihiko Osawa (Nagoya Univ.) and Dr. Yoji Kato (Univ. Hyogo). This antibody is specific for 3,5-Dibrominated tyrosine and other di-halogenated tyrosine at 3- and 5- position. Suitable for immunohistochemical analysys of oxidative stress.

Oxidative modification of tyrosine

Tyrosine is one of the main targets of protein oxidation, and it have been reported form nitrotyrosine, dityrosine and halogenated tyrosines depending on the type of free radicals. For example, hypochlorous acid (HOCl) and hypobromous acid (HOBr) are derived from neutrophil myeloperoxidase (MPO) and eosinophil peroxidase (EPO), and known to form dihalogenated tyrosines such as DiBrY, DiClY and DiIY. It is expected that DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.

Halogenated tyrosine related diseases

Cystic fibrosis:	VD Vliet, et.al.: Myeloperoxidase and protein oxidation in cystic fibrosis. Am J Physiol Lung Cell Mol Physiol. 279, L537-L546 (2000)
Atherosclerotic intima:	Hazen SL: 3-chlorotyrosine, a specific marker of myeloperoxidase ? catalyzed oxidation, is markedly elevated in low density lipoprotein isolated from human atherosclerotic intima.J Clin Invest 99, p2075-2081 (1997)
Sepsis:	Gaut JP, et.al.: Neutrophils employ the myeloperoxidase system to generate antimicrobial brominating and chlorinating oxidants during sepsis. PNAS 98, p11961-11966 (2001)
Asthma:	Wu W, et. al.: Eosinophils generate brominating oxidants in allergen - induced asthma. J Clin Invest 105, p1455-1463 (2000)

Specifications

Antigen:	3,5-dibromotyrosine conjugated KLH(keyhole limpet hemocyanin).
Form:	Lyophilized power. Reconstitute by 200 µL of distilled water. 100 µg/mL of IgG.
Subclass:	Mouse IgG_1(kappa), clone 3A5. Protein A purified.
Specificity:	This antibody can react with dibromotyrosine (DiBrY), N-acetylated dichloro tyrosine (NAcDiClY), diiodo tyrosine (DiIY), N-bromosuccinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosone conjugated BSA (halogene-modified tyrosine at both 3- and 5-position). Cross reactivity to following analogues have been tested: dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chrolotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3,4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. Weakly react with Bromotyrosine (BrY).
Application:	Immunohistochemistry, western blotting and ELISA.
Storage:	Less than -20°C

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Additional Information

Size:	20 ug of IgG/vial
Type of Marker:	Protein oxidation
Marker:	NO-modification
Storage:	Less than -20°C
Usage:	Tissue
Application:	Immunohistochemistry, western blotting and ELISA
Remark:	A novel nitrosative stress marker. Protein tryptophan residues are known to be the target of peroxynitrite to form tryptophan derivatives.