Anti-GAPDH Polyclonal Antibody | AB0067

Goat Anti-Human GAPDH IgG Polyclonal Antibody | AB0067 | Antibodies

Overview: Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase). GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Other Names: glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.

Other Names: glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.

Concentration: 2 mg/ml

Antibody Type: Primary

Target Type: Anti-GAPDH

Accession Number: ENSG00000111640

Host Species: Goat

Immunogen Species: Human

Immunogen Type: Recombinant protein

Immunogen: Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Isotype: IgG

Clonality: Polyclonal

Confirmed Reactive Species: Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Specificity Detail: Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Buffer: PBS, 20% glycerol and 0.05% sodium azide

Purification: Epitope affinity purified

Conjugation: Unconjugated

Format: N/A

Application: WB, IF, IHC-P, IHC-F

Concentration/Dilution: WB:1:500-1:10,000, IF:1:50-1:250, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000

Storage: N/A

References: 1. Broughton K, Korski K, Echeagaray O, et al. Gene Ther. 2019 Aug. PMID:31239537, 2. Korski KI, Kubli DA, Wang BJ, et al. Stem Cells. 2019 Jan. PMID:30629785, 3. Broughton K, Korski K, Echeagaray O, et al. bioRxiv 521732; Jan 2019, 4. Korski KI,MSc Thesis, Univ San Diego, USA 2018, 5. Choudhury SS, MSc Thesis, Univ San Diego, USA 2018, 6. Sacchi V, Wang BJ, Kubli D, et al. J Am Heart Assoc. 2017 Oct (Supplemental Information). PMID:29018025, 7. Khalafalla FG, Kayani W, Kassab A, et al. J Physiol. 2017 Oct. PMID:28980705, 8. Firouzi F, MSc Thesis, San Diego State University 2017, 9. Gonçalves A, Lin CM, Muthusamy A, et al. Invest Ophthalmol Vis Sci. 2016 May. PMID: 27163772, 10. Hyenne V, Apaydin A, Rodriguez D, et al. J Cell Biol. 2015 Oct. PMID: 26459596, 11. Helsby MA, Leader PM, Fenn JR. BMC Cell Biol. 2014 Feb. PMID: 24528853, 12. Grilo MLJ, MSc Thesis, University of Coimbra 2014, 13. Matos CTSM, PhD Thesis, NOVA University of Lisbon 2013