Anti- LIN28B Antibody | FNab04785

Anti- LIN28B Antibody | FNab04785 | Gentaur UK, US & Europe Distribution

Form: liquid

Purification: Immunogen affinity purified

Purity: ≥95% as determined by SDS-PAGE

Host: Rabbit

Clonality: polyclonal

Clone ID: N/A

Isotype: IgG

Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463) . Does not act on pri-miR21 (PubMed:22118463) . The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396) . This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463) . Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity) . When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Immunogen: lin-28 homolog B (C. elegans)

synonyms: CSDD2

Molecular weight (observed) : 27 kDa

Reactivity Species: Human, Mouse

Tested Application: ELISA, WB, IHC

Recomended Dillution : WB: 1:500-1:2000

Research Area: Epigenetics, Stem Cells, Metabolism