Anti-MERS-CoV S1 Mouse IgG2a Antibody | A2067

MERS-CoV belongs to the family of Coronaviridae and is characterized by large positive-sense RNA genome, that is 28 kb in length. One unique feature of MERS-CoV is that two-thirds of the viral genome is translated into two large polyproteins whereas the remaining viral genome is transcribed into sub-genomic RNAs. The two large polyproteins encode multiple non-structural proteins that play an important role in viral replication and transcription. MERS-CoV transcribes multiple sub-genomic RNAs that encode four structural proteins, namely, envelope (E), membrane (M), spike (S), and nucleocapsid (N). The Spike (S) protein is a 180 kDa type I glycosylated transmembrane protein, that assembles as a trimer on the surface of the virus, hence the coronavirus appears crown-shaped (In Latin, corona means crown). The ectodomain of the Spike protein consists of two domains: S1 domain whose main function is receptor binding, and S2 domain whose main function is membrane fusion. S1 binds to the cell surface receptor of host for attachment during the viral entry, this induces conformational change in the S2, thus enabling the fusion of host and viral membranes and ultimately entry of the viral genome in the host cell.

A2067 | Anti-MERS-CoV S1 Mouse IgG2a Antibody DataSheet

Antibody Target: MERS-CoV S1

Target Alternative Name:

Tag Line: Purified mouse monoclonal antibody to detect MERS Coronavirus Spike protein S1. This antibody does not have any crossreactivity with SARS Coronavirus

Category: Primary antibody

Host: Mouse

Isotype: IgG2a

Species Reactivities: Human

Immunogen Sequence:

Accession #:

Gene ID:

Appearance: Colorless liquid

Form: Liquid

Concentration:

Formulation: In 10 mM PBS, pH 7.2, 0.1% sodium azide

Purification: Affinity purified using protein A

Application: Immunofluorescence (IF),ELISA

Positive Control:

Application And Usages: ELISA, IF

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: False

Immunocytochemistry Verified: False

Immunofluorescence Verified: True

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: True

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: False