Anti Nitrotryptophan (NW) monoclonal antibody

Anti Nitrotryptophan (NW) monoclonal antibody

A novel biomarker for protein oxidation/nitration:

Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis, inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO-) is known as a potent biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide (O2-). Peroxinitrite can induce nitration of aromatic amino acids such as Tyr, Trp, Phe and His, which results in modulation of protein functions.

To date, 6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major ONOO--modified amino acids, and growing evidences suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress.
An anti-6-nitrotryptophan monoclonal antibody has been developed by Prof. Yamakura at Juntendo University, which is suitable for immunohistochemistry, western blotting and ELISA. This antibody may provide a powerful tool for the investigation of nitrosative stress inside living bodies.