Anti-SARS-CoV-2 Spike S1 Antibody | A3000

Coronaviruses (CoV) are a large group of enveloped positive-sense RNA viruses. They belong to subfamily Coronavirinae, in the family of Coronaviridae, of the order of Nidovirales. The Coronavirus genome is about 30 kb in length and encodes four structural proteins, namely, spike (S), envelope (E), membrane (M) and nucleocapsid (N), multiple nonstructural proteins and other accessory proteins. The Spike (S) protein is a 180 kDa type I glycosylated transmembrane protein, that assembles as a trimer on the surface of the virus, hence the coronavirus appears crown-shaped (In Latin, corona means crown). The ectodomain of the Spike protein consists of two domains: S1 domain whose main function is receptor binding, and S2 domain whose main function is membrane fusion. S1 binds to the cell surface receptor of host for attachment during the viral entry, this induces conformational change in the S2, thus enabling the fusion of host and viral membranes and ultimately entry of the viral genome in the host cell.

A3000 | Anti-SARS-CoV-2 Spike S1 Antibody DataSheet

Antibody Target: SARS-CoV-2 Spike S1

Target Alternative Name:

Tag Line: Rabbit polyclonal antibody to detect the expression of SARS-CoV-2 Spike Protein S1. This antibody can be match-paired with Anti-SARS-CoV-2 Spike S1 antibody (Clone# 4C6) (Catalog# A3001) for Sandwich ELISA

Category: Primary antibody

Host: Rabbit

Isotype: IgG

Species Reactivities:

Immunogen Sequence: CHO-expressed full length S1 with human IgG Fc fusion

Accession #:

Gene ID:

Appearance: Colorless liquid

Form: Frozen Liquid

Concentration:

Formulation: 0.01 M Tris-HCl, pH 8.0, 0.15 M NaCl, 0.02% sodium azide

Purification: Protein A purified

Application: Virus Neutralizing Assay,ELISA

Positive Control:

Application And Usages: Indirect ELISA (0.05 - 1 ?g/ml), Sandwich ELISA (0.25 - 1 ?g/ml) as coating antibody with Anti-SARS-CoV-2 Spike S1 antibody (Clone 4C6) (Catalog# A3001), Virus Neutralizing Assay (titer at 20 ?g/ml)

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: FALSE

Immunocytochemistry Verified: FALSE

Immunofluorescence Verified: FALSE

Immunoprecipitation Verified: FALSE

FACS Verified: FALSE

ELISA Verified: TRUE

ChIP Verified: FALSE

Dot Blot Verified: FALSE

Flow Cytometry Verified: FALSE