Anti-SHH Antibody | A1695

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

A1695 | Anti-SHH Antibody DataSheet

Antibody Target: SHH

Target Alternative Name: HHG 1,HHG-1,HHG1,HLP 3,HLP3,Holoprosencephaly 3,HPE 3,HPE3,MCOPCB5,shh,SHH,SMMC I,SMMCI,Sonic Hedgehog (Drosophila) homolog,sonic hedgehog homolog (Drosophila),Sonic hedgehog homolog,Sonic hedgehog protein,Sonic hedgehog protein C-product,TPT,TPTPS

Tag Line: Polyclonal antibody to detect endogenous levels of SHH in human, mouse and rat sample

Category: Stem cells

Host: Rabbit

Isotype: IgG

Species Reactivities: Rat,Mouse,Human

Immunogen Sequence: Synthetic peptide of human SHH

Accession #: Q15465

Gene ID: 6469

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/mL

Formulation: In PBS with 0.05% sodium azide, 50% glycerol, PH7.3

Purification: Affinity purification

Application: Immunohistochemistry (IHC),ELISA

Positive Control:

Application And Usages: ELISA,IHC 1:50-1:200

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Not Verified

Immunocytochemistry Verified: Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified: Not Verified

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified: Not Verified

Flow Cytometry Verified: Not Verified