Description
This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
A1695 | Anti-SHH Antibody DataSheet
Antibody Target: SHH
Target Alternative Name: HHG 1,HHG-1,HHG1,HLP 3,HLP3,Holoprosencephaly 3,HPE 3,HPE3,MCOPCB5,shh,SHH,SMMC I,SMMCI,Sonic Hedgehog (Drosophila) homolog,sonic hedgehog homolog (Drosophila),Sonic hedgehog homolog,Sonic hedgehog protein,Sonic hedgehog protein C-product,TPT,TPTPS
Tag Line: Polyclonal antibody to detect endogenous levels of SHH in human, mouse and rat sample
Category: Stem cells
Host: Rabbit
Isotype: IgG
Species Reactivities: Rat,Mouse,Human
Immunogen Sequence: Synthetic peptide of human SHH
Accession #: Q15465
Gene ID: 6469
Appearance: Colorless liquid
Form: Liquid
Concentration: 0.5 mg/mL
Formulation: In PBS with 0.05% sodium azide, 50% glycerol, PH7.3
Purification: Affinity purification
Application: Immunohistochemistry (IHC),ELISA
Positive Control:
Application And Usages: ELISA,IHC 1:50-1:200
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: Not Verified
Immunocytochemistry Verified: Verified
Immunofluorescence Verified: Not Verified
Immunoprecipitation Verified: Not Verified
FACS Verified: Not Verified
ELISA Verified: Not Verified
ChIP Verified: Not Verified
Dot Blot Verified: Not Verified
Flow Cytometry Verified: Not Verified
Additional Information
Size: |
100 uL |
Storage Condition: |
-20ºC |
Shipping Condition: |
Gel Pack |
Shelf Life: |
12 months |