Anti-Spastin Rabbit Monoclonal Antibody (RM346) | A1855

ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Severing activity is not dependent on tubulin acetylation or detyrosination. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex.

A1855 | Anti-Spastin Rabbit Monoclonal Antibody (RM346) DataSheet

Antibody Target: Spastin

Target Alternative Name:

Tag Line: Affinity purified Rabbit monoclonal to Spastin

Category: Primary antibody

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human

Immunogen Sequence: A peptide corresponding to the N-terminus of human Spastin.

Accession #: Q9UBP0

Gene ID: 6683

Appearance: Liquid

Form: Liquid

Concentration:

Formulation: 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Purification: Protein A affinity purified from an animal origin–free culture supernatant

Application: Western Blot: 1:500-1:1000, IHC: 1:500-1:1000

Positive Control:

Application And Usages: Western Blot: 1:500-1:1000, IHC: 1:500-1:1000

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: True

Immunocytochemistry Verified: True

Immunofluorescence Verified: False

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: False

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: False