AO | ascorbate oxidase, Biotin conjugated | AS09 383

AO | ascorbate oxidase, Biotin conjugated | AS09 383 | Gentaur UK, US & Europe Distribution

Immunogen: ascorbate oxidase purified from Cucurbita sp.

Host: Rabbit

Conjugation: Biotin

Clonality: Polyclonal

Isotype: N/A

Purity: Purified IgG in PBS pH 7.2.

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: AS06 184 | Anti-ascorbate oxidase rabbit antibodiesPlant and algal protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)

Molecular weight: 61 | 45 kDa (Cucurbita pepo)

Confirmed Reactivity: Cucurbita sp.

Predicted Reactivity: Glycne max, Oryza sativa Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: IgG concentration is 10 mg/ml, Biotin/IgG protein molar ratio is approximately 5, 6, No foreign proteins are added

Background: Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA) . Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Reconstitution: For reconstitution add 1 ml of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.

TAIR Nnumbre: N/A

Category: Oxidative stress

Research Area: Plant Biology