AP3B2 Antibody | 6395

AP3B2 Antibody | 6395 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: AP3B2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AP3B2.
The immunogen is located within the first 50 amino acids of AP3B2.

Research Area: Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: AP3B2 antibody can be used for detection of AP3B2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: Cat. No. 1403 – Mouse Brain Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 121 kDa
Observed: 128 kDa

Validation: N/A

Isoform: N/A

Purification: AP3B2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: AP3B2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: AP3B2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: AP3B2 Antibody: NAPTB, AP-3 complex subunit beta-2, Adapter-related protein complex 3 subunit beta-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: AP3B2 Antibody: The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2) , a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2) . The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Unlike the homologous AP3B1, AP3B2-containing AP3 complexes are preferentially targeted to neuronal processes, and mice deficient in AP3B2 displayed compromised synaptic zinc stores.