Apaf-1 Antibody [2E12] | 36-175

Apaf-1 Antibody [2E12] | 36-175 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Human

Homology: N/A

Immunogen: Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Research Area: Apoptosis, Cancer

Tested Application: E, ICC, IHC, IP, WB

Application: Immunohistochemistry: (paraffin sections, 10ug/ml) . Immunohistochemistry: (EM on frozen sections, see G. Hausmann, et al.; (2000) ) . Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.

Specificiy: Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: >95% (SDS-PAGE)

Clonality: Monoclonal

Clone: 2E12

Isotype: IgG2a, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. In PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: Apoptosis Protease-activating Factor 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD) , an ATPase domain (NB-ARC) , few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3) , leading to the activation of caspase-3 and apoptosis.