APEX1 Antibody | 45-009

APEX1 Antibody | 45-009 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow

Immunogen: The immunogen for this antibody is: PKRGKKGAVAEDGD-C

Research Area: Homeostasis

Tested Application: E, WB, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in nuclear lysates of cell lines A431, HeLa and MCF7 (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1 and NP_542380.1) . Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows nuclear staining of lobular epithelial cells. Recommended concentration: 4-6ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Specificiy: Reported variants represent identical protein (NP_001632.2; NP_542379.1; NP_542380.1) .

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: APE1, APEX1, APEX, APEX nuclease (multifunctional DNA repair enzyme) , APE, APX, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA- (apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic) , APEX nuclease 1 endonuclease, deoxyribonuclease (apurinic or apyrimidinic) , apurinic/apyrimidinic (abasic) endonuclease, redox factor 1

User Note: N/A

BACKGROUND: N/A