ApoJ/Clusterin, human recombinant | 7584

Native Apolipoprotein J (ApoJ), also named Clusterin, is a heavily glycosylated, 75-80 kDa disulfide-linked heterodimeric protein. Despite being cloned since 1989, no genuine function has been attributed to ApoJ so far. The protein has been reportedly implicated in several diverse physiological processes such as sperm maturation, lipid transportation, complement inhibition, tissue remodeling, membrane recycling, cell-cell and cell-substratum interactions, stabilization of stressed proteins in a folding-competent state and promotion or inhibition of apoptosis. ApoJ gene is differentially regulated by cytokines, growth factors and stress-inducing agents. Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.

7584 | ApoJ/Clusterin human recombinant DataSheet

Biomolecule/Target: ApoJ/Clusterin

Synonyms: CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, ApoJ

Alternates names: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1, Alpha platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor

Taglines: A heavily glycosylated protein upregulated in multiple pathological conditions

NCBI Gene ID #: 5154

NCBI Gene Symbol: PDGFA

Gene Source: Human

Accession #: P04085

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): The chaperone activity of ApoJ was confirmed by its ability to bind to proteins under physical stress such as heat (e.g. Thyroglobulin) and chemical stress such as change in pH (e.g. Procathepsin K).

Biological activity: The ED as determined by the dose-dependent stimulation of thymidine uptake by Balb-c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of >1 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 28.5 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 2 mg/ml solution of ApoJ in 25 mM Na2HPO4 and 100 mM NaCl (pH 7.5).

Reconstitution Instructions: Reconstitution in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A