Description
APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. Recombinant murine APRIL is a soluble 21.9 kDa protein, consisting of 192 amino acid residues.
7118 | APRIL Mouse Recombinant DataSheet
Biomolecule/Target: APRIL
Synonyms: A Proliferating-inducing Ligand, TNFSF13, TRDL-1
Alternates names: Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor polypeptide, Becaplermin.
Taglines: Stimulates proliferation of various tumor cell lines like Jurkat T cells and MCF-7 carcinoma cells.
NCBI Gene ID #: 5155
NCBI Gene Symbol: PDGFB
Gene Source: Mouse
Accession #: P01127
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to induce cell proliferation of activated T-cells.
Biological activity: The ED as determined by the dose-dependent stimulation of thimidine uptake by BALB/c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of > 1 x 10 units/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 24.3 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 5 and 100 mM Arginine.
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A