AQP2 Antibody | 7621

AQP2 Antibody | 7621 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Sheep: (100%) , Bovine: (95%)

Immunogen: AQP2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AQP2.
The immunogen is located within the last 50 amino acids of AQP2.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: AQP2 antibody can be used for detection of AQP2 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: AQP2 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with other members of the aquaporin protein family.

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 30 kDa
Observed: 28 kDa

Validation: N/A

Isoform: N/A

Purification: AQP2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: AQP2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: AQP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: AQP2 Antibody: AQP-CD, WCH-CD, Aquaporin-2, ADH water channel, AQP-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Aquaporins are membrane proteins that serve in the transfer of water and small solutes across cellular membranes. One such aquaporin, aquaporin-2 (AQP2) is located in the kidney collecting tubule and plays a critical role in water reabsorbtion (1) . AQP2 is mainly localized in intracellular vesicles but upon stimulation with anti-diuretic hormone (ADH) , AQP2 is translocated to the apical plasma membrane by exocytic fusion of AQP2-bearing vesicles (2) . Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus (3) .