ARF1 | ADP-ribosylation factor 1 | AS08 325

ARF1 | ADP-ribosylation factor 1 | AS08 325 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant GST fusion of full length of Arabidopsis thaliana ARF1 (P36397, AT2G47170)

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunofluorescence (IF), Immunogold (IG), Western blot (WB)

Related Products: AS08 327 | Anti-Sec21 (gamma subunit, COP vesicles) (Golgi marker in immunolocalization and COP1 marker in western blot), rabbit antibodiesAS08 325PRE | Arf1 | ADP-ribosylation factor 1, pre-immune serumrecommended secondary antibodyPlant and algal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)

Molecular weight: 21 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Elaeis sp., Lilium longiflorum, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Petunia hybrida cv Mitchell, Physcomitrium patens, Solanum tuberosum

Predicted Reactivity: Brassica juncea, Brassica napus, Capsella rubella, Capsicum annum, Cucumis sp., Daucus carota, Elaeis guineensis, Glycine max, Helleborus orientalis, Hordeum vulgare, Medicago truncatula, Nannochloropsis gaditana, Nicotiana benthamina, Ostreococcus tauri, Populus trichocarpa, Zea maysSpecies of your interest not listed? Contact us

Not reactive in: Microsporidia sp.

Additional Information: References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000) . In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12 (11) :2219-36.Ritzenthaler et al. (2002) . Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14 (1) :237-61.

Background: The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G47170

Category: Endomembrane system

Research Area: Cell Biology