ARNTL (Human) ELISA Kit - E4990

E4990 | ARNTL (Human) ELISA Kit DataSheet

Aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL, BMAL1) is a basic helix-loop-helix transcription factor with 2 PAS domains. It is a critical component of the circadian clock. ARNTL contributes to the positive limb of the transcription-translation feedback loop. It heterodimerizes with CLOCK and induces the expression of downstream circadian clock genes Periods (Pers) and Cryptochromes (Crys) inside the nucleus. Upon synthesis in the cytoplasm, PER and CRY proteins bind to Casein Kinases (CKs), translocate back to the nucleus, and inhibit CLOCK-ARNTL transcriptional activity. ARNTL also regulates hypertension, diabetes, and obesity. Mutations and knockouts of ARNTL are associated with infertility, impaired glucose and lipid homeostasis, disturbed sleep patterns, reduced life span, and age-related pathologies. ’s ARNTL (Human) ELISA kit quantitatively measures ARNTL in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ARNTL in the sample or standard.

Alternate Name : TIC, JAP3, MOP3, BMAL1, PASD3, BMAL1c, bHLHe5, ARNTL

Tag Line : A Sandwich ELISA kit for quantitative measurement of ARNTL in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Other biological fluids Tissue lysate Serum Plasma

Species Reactivity : Human

Applications : Sandwich ELISA kit to quantitatively measure ARNTL in human serum, plasma, tissue lysate, and other biological fluids

Features and Benefits : Recovery range: 85 - 105% for normal human serum and plasma samples This Sandwich ELISA is highly sensitive and highly specific for the detection of ARNTL in human samples. There is no significant cross-reactivity or interference between ARNTL and analogues Detection range: 15.625 – 1000 pg/ml Sensitivity: 9.375 pg/ml Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%