Arthrogen-CIA 5-Clone Cocktail Kit - 40mg

Arthrogen-CIA 5-Clone Cocktail Kit - 40mg - Cat Number: 53040 From .

Research Field: Arthritis

Clonality: Monoclonal

Cross-Reactivity:

Host Origin: Mouse

Applications: N/A

Isotype: IgG2a/2b

Detection Range: N/A

Sample Type: N/A

Concentration: 10 mg/ml

Immunogen:

PRODUCT SPECIFICATIONS

DESCRIPTION: Mouse anti-type II collagen 5-clone monoclonal antibody cocktail kit with LPS from E. Coli
O111:B4
APPLICATION: Use for collagen antibody induced arthritis (CAIA) in mice
QUANTITY: Cocktail: 10 mg/ml x 4 ml
LPS: 0.5 mg/ml x 3.5 ml
FORM: A cocktail of 5 monoclonal antibodies dissolved in 0.05M phosphate buffered saline, pH 7.4
which recognize the conserved epitopes on various species of type II collagen: clone A2-10
(IgG2a), F10-21 (IgG2a), D8-6 (IgG2a), D1-2G (IgG2b), and D2-112 (IgG2b). Clones A2-10,
D1-2G, and D2-112 recognize individual epitopes clustered within the 167 amino acid peptide
fragment called LyC1 (124-290) of the CB11 fragment (124-402). Clones F10-21 and D8-6
recognize epitopes within the 83 amino acid peptide fragment called LyC2 (291-374) of the
CB11 fragment.
SOURCE: Mouse
CROSS-REACTIVITY: Cross-reacts to most species of type II collagen including mouse, porcine, chick*, bovine,
human, rat, monkey, rabbit, equine, and dog.
*D2-112 does not cross-react with chick type II collagen.

ANIMALS: 7-8 weeks or older, high responder mice: DBA/1, Balb/c, B10.RIII, C.B-17, scid/scid, or 129/Sv. Low responder mice: C57BL/6 or C57BL/6 background. Sensitivity to CAIA may vary depending on mouse vendor and facility. Specific pathogen free (SPF) housing conditions are strongly recommended over conventional housing conditions as bacterial contamination may reduce the immune response of the animals resulting in an attenuated arthritis. Therefore, running a pilot study using a small number of animals before conducting a large-scale study is strongly recommended. Please contact , Inc. customer support (support@.com) for
guidance regarding animals and housing conditions.

USAGE: Administration of the monoclonal antibody cocktail by IV injection (i.e. tail vein) is recommended;
however, intraperitoneal (IP) injection may also be used. Moreover, if LPS use is not desired,
please note that the severity of arthritis tends to be lower.

PROTOCOLS:

A. Inducing arthritis with a combination of the monoclonal antibody cocktail and LPS in CAIA
susceptible mice (DBA/1, Balb/c, B10.RIII, C.B-17, scid/scid, or 129/Sv)
           Day 0: Inject 1.5 mg of 5-clone cocktail by IV or IP injection.
           Day 3: Inject 25-50 μg of LPS by IP injection.

           NOTE: Moderate to severe arthritis is observed on day 3-4 and peaks around day 7-10.
           B. Inducing arthritis with a combination of the monoclonal antibody cocktail and LPS in CAIA
           low responder mice (C57BL/6 or C57BL/6 background)
           Day 0: Inject 5 mg of 5-clone cocktail by IV or IP injection.
           Day 3: Inject 25-50 μg of LPS by IP injection.
           NOTE: Moderate to severe arthritis is observed on day 3-4 and peaks around day 7-10.
           An injection of LPS on day 10-14 can be used to re-stimulate inflammatory arthritis.
           C. Inducing arthritis with the monoclonal antibody cocktail without LPS in DBA/1 and Balb/c
           mice
           Day 0: Inject 6-10 mg of 5-clone cocktail by IV injection.
           NOTE: Arthritis can be observed on day 2-3. This protocol has not been confirmed in other
           CAIA susceptible mouse strains such as B10.RIII, C.B-17, scid/scid, or 129/Sv mice.
STORAGE: -80°C
STABILITY: 2 years
NOTES: N/A
REFERENCES: K. Terato et al. J. Immunol. 148: 2103-2108 (1992)
                     K. Terato et al. Autoimmunity 22: 137-147 (1995)
                     S. Yoshino et al. J. Immunol Methods 343: 49-55 (2009)