ATG7 Antibody (Ascites) [APR07072G]

ATG7 Antibody (Ascites) [APR07072G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: Purified His-tagged APG7 protein was used to produced this monoclonal antibody.

Cellular Localisation: Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme

Molecular Weight: 77960

Clone: Monoclonal

Gene Name: ATG7

Gene ID: 10533

Function: E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity) .

Summary: Tissue Location: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

Form: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:500~2000

Synonyms: Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, APG7-like, hAGP7, Ubiquitin-activating enzyme E1-like protein, ATG7, APG7L