ATMIN Antibody | 7777

ATMIN Antibody | 7777 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: ATMIN antibody was raised against a 19 amino acid peptide near the amino terminus of human ATMIN.
The immunogen is located within amino acids 200 - 250 of ATMIN.

Research Area: Stem Cell

Tested Application: E, WB, ICC, IF

Application: ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: ATMIN antibody is human, mouse and rat reactive. At least three isoforms of ATMIN are known to exist.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 17-002 - A-431 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 73, 79, 91 kDa
Observed: 72 kDa

Validation: N/A

Isoform: N/A

Purification: ATMIN antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ATMIN Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ATMIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: ATMIN Antibody: ASCIZ, ZNF822, KIAA0431, ATM interactor, Zinc finger protein 822, ASCIZ

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The ATM/ATR-substrate CHK2-interacting zinc finger protein (ATMIN) , also known as ASCIZ, forms DNA damage-induced nuclear foci that contain the DNA repair protein Rad51 (1) . ATMIN is also thought to be involved in embryonic development, as an absence of ATMIN causes late-embryonic lethality in mice with a range of organ development defects (2) . It also activates the transcription DYNLL1, a light chain of the dynein motor complex and sequence-specific regulator of protein dimerization of numerous targets. DYNLL1 can bind to and inhibit the transcription activation domain of ATMIN, forming a simple dynamic feedback loop for DYNLL1 expression (3) .