Description
ATMIN Antibody | 7777 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: ATMIN antibody was raised against a 19 amino acid peptide near the amino terminus of human ATMIN.
The immunogen is located within amino acids 200 - 250 of ATMIN.
Research Area: Stem Cell
Tested Application: E, WB, ICC, IF
Application: ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: ATMIN antibody is human, mouse and rat reactive. At least three isoforms of ATMIN are known to exist.
Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2: Cat. No. 17-002 - A-431 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 73, 79, 91 kDa
Observed: 72 kDa
Validation: N/A
Isoform: N/A
Purification: ATMIN antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ATMIN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: ATMIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: ATMIN Antibody: ASCIZ, ZNF822, KIAA0431, ATM interactor, Zinc finger protein 822, ASCIZ
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The ATM/ATR-substrate CHK2-interacting zinc finger protein (ATMIN) , also known as ASCIZ, forms DNA damage-induced nuclear foci that contain the DNA repair protein Rad51 (1) . ATMIN is also thought to be involved in embryonic development, as an absence of ATMIN causes late-embryonic lethality in mice with a range of organ development defects (2) . It also activates the transcription DYNLL1, a light chain of the dynein motor complex and sequence-specific regulator of protein dimerization of numerous targets. DYNLL1 can bind to and inhibit the transcription activation domain of ATMIN, forming a simple dynamic feedback loop for DYNLL1 expression (3) .